We had the privilege of participating in the Neuro-Immune Axis: Reciprocal Regulation in Development, Health, and Disease conference held in Long Beach, California, USA from September 22-14, 2019. The Cell Symposia are compact scientific meetings organized by Cell Press and Elsevier that highlight cutting-edge research and provide networking with experts in the field. The goal of the symposium is to explore the interface between the nervous and immune systems during development, homeostasis, and disease.
Sunil Gandhi, Ph.D. our Co-Founder, presented a poster titled “Brain-wide imaging of neuroinflammation.” Resident and infiltrating immune cells play region-specific roles in neurological diseases that are poorly understood. Fortunately, new advances in light sheet imaging and whole brain clearing have opened an exciting new avenue for brain wide visualization of neuroinflammation. At Translucence Biosystems, we have harnessed these techniques to develop a rapid yet precise method for brain-wide detection of neuroinflamation. We have successfully reduced the imaging time of a whole mouse brain to less than 25 min while preserving fine anatomical detail.
Our participation in the Neuro-Immune Axis conference provided a platform to showcase our groundbreaking work and foster collaborations with esteemed researchers. Explore how our science, services, and products are paving the path for next-generation 3D histology.
To learn more about our research, we encourage you to access the poster presented at the Neuro-Immune Axis conference below, and refer to our abstract.
Abstract: Resident and infiltrating immune cells play region-specific roles in neurological diseases that are poorly understood. New advances in light sheet imaging and whole brain clearing have opened an exciting new avenue for brain wide visualization of neuroinflammation. We have dramatically sped up light sheet imaging of whole mouse brain samples to take less than 3 hours while preserving fine anatomical detail. Using machine-learning algorithms, we extract 3D profiles of immunolabeled microglia and monocytes. Alignment to the Allen reference atlas enables the unbiased, regionalized analysis of cellular patterns across >1200 brain areas. Using our rapid, brain wide detection assay reveals intriguing brain circuit specific patterns of monocyte infiltration specific to Toxoplasma infection. We have also applied this technology to visualize microglial proliferation and morphological activation in response to a widely studied set of immunological stimuli. We have extended our approach to the visualization of microglia across the rat brain. Our unbiased approach to the detection of neuroinflammation holds promise for the generation of new circuit specific hypotheses for interactions at the neuro-immune interface.
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