Light Sheet Image Stitching

Custom stitching jobs of large terabyte-sized datasets using Stitchy

Modular and Customizable

Light Sheet Stitching is the third step of our modular custom service offerings, delivering complete 3D datasets of clients’ native lightsheet files.

Background

Handling large terabyte-sized datasets can be challenging with standard stitching pipelines. To streamline the light sheet stitching workflow, our internal team has developed Stitchy

Stitchy converts data from your native microscope file type to your preferred format, ensuring seamless integration with downstream quantification applications. 

Learn more about light sheet stitching on our science page.

Background

Simply send us your raw light sheet files, and we’ll return the output in your preferred format.

Supported File Types:

  • ZEISS Lightsheet Z.1
  • ZEISS Lightsheet 7
  • Miltenyi UMII and Blaze
  • Life-Canvas Tech

Supported Output Formats:

  • Imaris
  • OME-Tif as BigTIFF
  • TIFF Sequence
  • NGFF

We also support multi-view and dynamic focus data in the formats listed above.

If your file type is not on the supported list, let’s collaborate. We are continually striving to increase compatibility and would like to work with you. Please schedule a 15-minute call with our team or reach out to us at info@translucence.bio.

Light Sheet Image Stitching

Light Sheet Stitching is the third step of our modular custom service offerings, delivering complete 3D datasets of clients’ native lightsheet files.

Simply send us your raw light sheet files, and we’ll return the output in your preferred format.

Supported File Types:

  • ZEISS Lightsheet Z.1
  • ZEISS Lightsheet 7
  • Miltenyi UMII and Blaze
  • Life-Canvas Tech

Supported Output Formats:

  • Imaris
  • OME-Tif as BigTIFF
  • TIFF Sequence
  • NGFF

We also support multi-view and dynamic focus data in the formats listed above.

If your file type is not on the supported list, let’s collaborate. We are continually striving to increase compatibility and would like to work with you. Please schedule a 15-minute call with our team or reach out to us at info@translucence.bio.

3D-Stitched Images Gallery

3D-Stitched Images Gallery

Browse actionable insights generated by the

Light Sheet Image Stitching

service

Browse through images of some of the lightsheet images and videos that have been generated by our Custom Light Sheet Stitching services

Whole Mouse Brain Stained With Smooth Muscle Actin

Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.

Coronavirus-Induced Spinal Cord Neuroinflammation

This spinal cord sample was prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.

Kidney Smooth Muscle Actin

This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.

Tyrosine Hydroxylase (TH) Staining of a Whole Mouse Brain

Tyrosine Hydroxylase (TH) staining of a whole mouse brain visualizes the distribution of dopamine, norepinephrine, and epinephrine-containing (catecholamine) neurons and endocrine cells. TH is a key enzyme involved in neurotransmitter production. Immunohistochemical detection of TH expression is one tool for visualizing and quantifying damage and loss of dopaminergic neurons, involved in disorders including Parkinson’s disease. Sample imaging performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.

Interested in Light Sheet Stitching solutions?

Light Sheet Stitching delivers complete 3D datasets of your native light sheet files.

Interested in performing stitching in-house? Stitchy™ is a light sheet image stitching tool that streamlines your light sheet stitching workflow– performing 2.5-5.5x faster than leading commercial software solutions.
Learn more about Stitchy™ →

Heading

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Suspendisse varius enim in eros elementum tristique. Duis cursus, mi quis viverra ornare, eros dolor interdum nulla, ut commodo diam libero vitae erat. Aenean faucibus nibh et justo cursus id rutrum lorem imperdiet. Nunc ut sem vitae risus tristique posuere.

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

Heading 1

Heading 2

Heading 3

Heading 4

Heading 5
Heading 6

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.

Block quote

Ordered list

  1. Item 1
  2. Item 2
  3. Item 3

Unordered list

  • Item A
  • Item B
  • Item C

Text link

Bold text

Emphasis

Superscript

Subscript

Request a quote

Please select which services are you interested in we will reach out to you shortly

Custom services

Contact information

Back
Next

Thank you!

Your submission has been received

Something went wrong while submitting the form.
Please, contact us via email info@translucencebio.com