Try
Try our kit today
Your Pathway to Brain-Wide Cellular Resolution Monitoring of Neuronal Activity
Capture comprehensive snapshots of recent neuronal activity patterns across the entire brain by measuring the expression of immediate-early gene (IEG) products, Npas4 and cFos, at single-cell resolution.
The drug-treated mouse brain above, cleared with the Neuronal Activity Tissue Clearing Kit, shows Npas4 immunoreactivity in green and cFos immunoactivity in red. Each kit includes validated antibodies for Npas4 and cFos so that you can obtain cellular-resolution brain-wide snapshots of neuronal activity in your own lab– no experience or specialized lab equipment required.
A straightforward solution for simultaneous measurement of cFos and Npas4 throughout the brain, allowing for cellular-resolution monitoring of recent neuronal activity-dependent signal transduction.
User-friendly tissue clearing protocol delivering industry quality results
Validated cFos and Npas4 antibodies
Cellular-resolution monitoring of recent neuronal activity
Translucence Biosystems is dedicated to partnering with researchers on their path to discovery. We recognize the critical role that three-dimensional analysis of biological samples plays in advancing scientific research.
That is why we have developed a comprehensive approach to 3D histology, integrating innovative tissue clearing techniques, state-of-the-art imaging technologies, and advanced quantification pipelines.
These solutions enable rapid, reliable analysis of intact samples, revealing insights often overlooked by traditional 2D slice IHC methods.
The Neuronal Activity Tissue Clearing Kit enables researchers to capture activity patterns across the entire brain by measuring the expression of immediate-early genes (IEGs), Npas4 and cFos, at single-cell resolution.
Although cFos is widely used as a marker of neuronal activity, its expression is also associated with other signaling pathways such as cAMP elevations, neurotrophins, and other paracrine factors, which reduces its specificity as a marker of pure neuronal activity. In contrast, Npas4 is precisely regulated by Ca2+-dependent signaling downstream of neuronal activity.
The divergent nature of these two IEG products, as shown in the figure to the left, highlights the importance of measuring both gene products to gain a comprehensive understanding of the brain-wide signatures associated with neuronal activity and other signaling mechanisms in response to genetic, pharmacological, or behavioral manipulations.
Furthermore, the figure above showcases zoomed-in regions from 2D optical slices at various plane depths in a single brain, demonstrating the high resolution and antibody penetration one can attain with the Translucence Neuronal Activity Tissue Clearing Kit.
Our protocol calendar breaks down the clearing kit process into manageable steps, so you can keep track of progress and stay on schedule.
We know that time is of the essence when it comes to your research. That's why we've created a handy protocol calendar that lays out the Neuronal Activity Tissue Clearing Kit Protocol in a clear, easy-to-follow format.
For detailed information on the Neuronal Activity Tissue Clearing Kit Protocol refer to our product manual on our support page, An abridged version of the protocol can also be viewed.
Reagents and Disposables for 5 Whole Mouse Brains:
— 10x Washing Buffer 1;
— 10x Washing Buffer 2;
— Permeabilization Solution;
— Blocking Solution;
— Primary AB Buffer;
— Secondary AB Buffer;
— Anti-Npas4 Antibody;
— Anti-cFos Antibody;
— Npas4 Secondary Antibody (568);
— cFos Secondary Antibody (647);
— Sample Tubes included.
Our protocol calendar outlines the clearing process in manageable steps, helping you track progress and maintain your research schedule efficiently.
Our protocol overview and user-friendly protocol allow you to accurately assess workload and optimize your time effectively.
Reagents and Disposables for 5 Whole Mouse Brains
10x Washing Buffer 1
10x Washing Buffer 2
Permeabilization Solution
Blocking Solution
Primary AB Buffer
Secondary AB Buffer
Anti-Npas4 Antibody
Anti-cFos Antibody
Npas4 Secondary Antibody (568)
cFos Secondary Antibody (647)
Sample Tubes
Our ecosystem of 3D histology solutions is designed to accelerate your research process and help you achieve your research goals.
In addition to our products and services, we are proud to offer a team of expert scientists to provide support and guidance throughout your path to discovery.
At Translucence Biosystems, we are not just a provider of 3D histology solutions– we are a partner in your research success.
Capture comprehensive snapshots of recent neuronal activity across the entire brain by measuring the expression of immediate-early gene products Npas4 and cFos at single-cell resolution.
We’d like to answer your tissue clearing questions and provide recommendations to support your existing workflow
