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Visualization of the vasculature network within various tissue types
Blood vessels, the transport network of biological tissues, are instrumental in the delivery of therapeutics and essential molecules.
Smooth-Muscle Actin (SMA), a marker of vascular smooth muscle cells, facilitates visualization of the vasculature network within various tissue types.
Understanding and visualizing vasculature facilitates a better understanding of the mechanisms of therapeutic delivery and tissue function. This makes them a target for studies aiming to detect changes in the 3D network-like structure of vasculature. Learn more about our work with vasculature on our science page →
The Vascular Staining service focuses on whole-brain vasculature staining. Importantly, we have validated SMA-mediated vasculature staining in other organs, including mouse liver, brain, lung, and kidney.
3D Visualization of Vasculature Networks
Perform your favorite behavioral, pharmacological, genetic, or other manipulation, and send us your samples. Or collaborate with us, and we’ll perform the animal treatments for you.
The Vasculature Staining service leverages Translucence Biosystems’ modified iDISCO+ tissue clearing protocol to render intact brain samples optically transparent. Staining for Smooth-Muscle Actin (SMA), CD31, and Podocalyxin reveals the arteries, veins, and capillaries across the whole brain.
Cleared, labeled samples are imaged on the Lightsheet Z.1 equipped with the Mesoscale Imaging System™. The Mesoscale Imaging System™ is optimized for imaging large intact tissues with unprecedented speed and resolution and is compatible with the high refractive index solutions used in tissue clearing protocols, such as iDISCO+.
Translucence’s imaging pipelines capture cellular-resolution, whole-sample images of vascular networks, providing insights into the 3D network-like structures in your tissue of interest.
Translucence Biosystems employs our in-house developed software solution, Stitchy, for automated light sheet image stitching. Stitchy is a robust software solution that stitches large imaging datasets 2.5-5.5 times faster than leading commercial software solutions. Stitchy enables seamless integration with downstream image analysis pipelines by eliminating intermediate file formats.
Browse actionable insights generated by the
Whole-Organ Vasculature Staining
service
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
This image shows a mouse brain stained for smooth muscle cells surrounding arteries using tissue-clearing and immunolabeling methods. This helps scientists visualize the blood-brain barrier, a critical barricade that limits the entry of therapeutics into the brain. This image was featured in the 2024 BRAIN Initiative Calendar.
Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using theTranslucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.
This image shows a mouse brain stained for smooth muscle cells surrounding arteries using tissue-clearing and immunolabeling methods. This helps scientists visualize the blood-brain barrier, a critical barricade that limits the entry of therapeutics into the brain. This image was featured in the 2024 BRAIN Initiative Calendar.
Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using theTranslucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.
The Whole-Organ Vasculature Staining service leverages next-generation 3D histology technologies including tissue clearing and light sheet imaging to visualize vasculature.
This service is suited for studies aiming to gain a better understanding of the mechanisms of therapeutic delivery and tissue function, or for studies aiming to detect changes in the 3D network-like structure of vasculature as a function of experimental manipulation.
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