3D Tissue Clearing

Custom tissue clearing solutions using the Translucence modified iDISCO+ protocol or protocols of your choice.

Modular and Customizable

3D Tissue Clearing is the first step of our modular custom service offerings, providing users with precise and robust clearing tailored to their specific tissue, epitope, and protocol requirements.

Background

For hundreds of years, the diffraction of light has prevented seeing deep into biological specimens, requiring cutting thin slices to view on microscope slides. Recent advances in tissue clearing have solved this problem, opening up profound insights into the three-dimensional architecture of tissues, allowing for light sheet imaging of labeled objects throughout intact samples. Translucence Biosystems is committed to closely collaborating with researchers and applying our advanced tissue clearing methodologies to address your specific biological questions.

Learn more about tissue clearing on our science page.

Background

In addition to our in-house modified iDISCO+ protocol, we can implement your preferred clearing method or collaborate with you to develop new clearing and staining protocols.

We can clear and stain any number of samples, support a wide variety of sample types, and provide insights into optimal sample usage based on your experimental manipulations.

Supported Protocols:

  • Translucence Biosystems modified iDISCO+ protocol
  • Adipoclear
  • EZ Clear
  • Others

Validated Sample Types:

  • Brains (Mouse, Rat, Tree Shrew)
  • Spinal Cords
  • Kidneys
  • Lungs
  • Liver
  • Pancreas
  • Embryo
  • Tumors

Some Validated Assays of Interest:

  • Smooth Muscle Actin
  • Npas4
  • cFos
  • GFP
  • CK7
  • TH
  • Iba1
  • β-Amyloid
  • Anti-Human Secondary (human targets)
  • CD31/Podocalyxin
  • ɑ-Synuclein
  • PV

If you don’t see your protocol, sample type or epitope of interest, please contact our team at info@translucencebio.com, for additional support or custom protocols.

3D Tissue Clearing

3D Tissue Clearing is the first step of our modular custom service offerings, providing users with precise and robust clearing tailored to their specific tissue, epitope, and protocol requirements.

In addition to our in-house modified iDISCO+ protocol, we can implement your preferred clearing method or collaborate with you to develop new clearing and staining protocols.

We can clear and stain any number of samples, support a wide variety of sample types, and provide insights into optimal sample usage based on your experimental manipulations.

Supported Protocols:

  • Translucence Biosystems modified iDISCO+ protocol
  • Adipoclear
  • EZ Clear
  • Others

Validated Sample Types:

  • Brains (Mouse, Rat, Tree Shrew)
  • Spinal Cords
  • Kidneys
  • Lungs
  • Liver
  • Pancreas
  • Embryo
  • Tumors

Some Validated Assays of Interest:

  • Smooth Muscle Actin
  • Npas4
  • cFos
  • GFP
  • CK7
  • TH
  • Iba1
  • β-Amyloid
  • Anti-Human Secondary (human targets)
  • CD31/Podocalyxin
  • ɑ-Synuclein
  • PV

If you don’t see your protocol, sample type or epitope of interest, please contact our team at info@translucencebio.com, for additional support or custom protocols.

3D-Cleared Tissue Gallery

3D-Cleared Tissue Gallery

Browse actionable insights generated by the

3D Tissue Clearing

service

Browse through images of some of the cleared tissues that have been generated by our Custom 3D Tissue Clearing services

Whole Mouse Brain Stained With Smooth Muscle Actin

Vascular network of a mouse brain, revealed with Smooth Muscle Actin (SMA) staining. The sample was prepared using our NIH BRAIN Initiative-funded tissue clearing kits, designed for clearing and staining various biological tissues. Imaging performed on the ZEISS Lightsheet Z.1 equipped with our Mesoscale Imaging System.

Coronavirus-Induced Spinal Cord Neuroinflammation

This spinal cord sample was prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.

Kidney Smooth Muscle Actin

This kidney sample was stained with Smooth Muscle Actin (SMA) and prepared using Translucence Universal Tissue Clearing Kit, which in combination with your chosen antibodies, empowers you to effortlessly clear and stain an extensive range of tissues, including mouse brain, lung, liver, spinal cord, and more.

Tyrosine Hydroxylase (TH) Staining of a Whole Mouse Brain

Tyrosine Hydroxylase (TH) staining of a whole mouse brain visualizes the distribution of dopamine, norepinephrine, and epinephrine-containing (catecholamine) neurons and endocrine cells. TH is a key enzyme involved in neurotransmitter production. Immunohistochemical detection of TH expression is one tool for visualizing and quantifying damage and loss of dopaminergic neurons, involved in disorders including Parkinson’s disease. Sample imaging performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.

Liver Vasculature

Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.

Arteries: A Brain Imaged With Heart

This image shows a mouse brain stained for smooth muscle cells surrounding arteries using tissue-clearing and immunolabeling methods. This helps scientists visualize the blood-brain barrier, a critical barricade that limits the entry of therapeutics into the brain. This image was featured in the 2024 BRAIN Initiative Calendar.

Embryonic β-Tubulin Staining

Embryonic β-Tubulin staining showcases the intricate structure of a mouse embryo. This footage is brought to light using Light Sheet Fluorescence Microscopy (#LSFM) techniques, imaged on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™️. Sample preparation performed using our NIH-funded Universal Tissue Clearing Kit.

iDISCO Whole-Organ Imaging of Intact Mouse Liver

Whole mouse liver stained with Smooth Muscle Actin (SMA) in Cyan and Cytokeratin 7 (CK7) in green. Tissue clearing and staining for SMA and CK7 enables the use of Light Sheet Fluorescence Microscopy (LSFM) to visualize vasculature and bile duct networks – providing comprehensive insights into the 3D morphology of both targets.

Interested in 3D Tissue Clearing?

3D Tissue Clearing provides users with precise and robust clearing tailored to their specific tissue, epitope, and protocol requirements.

For those looking to perform tissue clearing in-house, our NIH-funded tissue clearing kit offerings provide a straightforward path to expert-level clearing and staining.

NIH-funded tissue clearing kit users are eligible for a discounted rate on AI-Powered 3D Quantification services to assist in your sample analysis.

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