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3D Bile-Duct Visualization and Quantification
Bile ducts located in the liver play a crucial role in the delivery of bile. Many liver diseases contribute to morphological abnormalities within the branching networks of liver bile ducts. Given this, visualizing abnormalities in liver bile duct morphology is crucial to designing robust therapeutic interventions.
The Full-Pipeline Liver Bile Duct service targets Cytokeratin 7 (CK7) throughout the intact liver. This service is well suited for studies investigating the complex branching networks of liver bile ducts. 3D visualization allows this service to provide comprehensive insights into morphological changes induced by genetic or pharmacological manipulations.
Learn more about the advantages of 3D bile-duct visualization and quantification on our science page →
3D Bile-Duct Visualization and Quantification
Perform your favorite behavioral, pharmacological, genetic, or other manipulation, and send us your samples. Or collaborate with us, and we’ll perform the animal treatments for you.
The Liver Bile Duct and Other Tubular Structures Quantification service utilizes Translucence Biosystems’ modified iDISCO+ tissue clearing protocol to render intact samples optically transparent. Staining for Cytokeratin 7 (CK7), found in liver bile ducts and Smooth Muscle Actin (SMA), a marker of vascular smooth muscle cells, facilitates the visualization of the bile ducts and vasculature across the intact liver.
Cleared, labeled samples are imaged on the Lightsheet Z.1 equipped with the Mesoscale Imaging System™. The Mesoscale Imaging System™ is optimized for imaging large intact tissues with unprecedented speed and resolution and is compatible with the high refractive index solutions used in tissue clearing protocols, such as iDISCO+.
Translucence’s imaging pipelines capture cellular-resolution, whole-sample images of liver bile ducts and other tubular structures, providing comprehensive insights into morphological changes induced by genetic or pharmacological manipulations.
We employ our in-house developed software solution, Stitchy, for automated light sheet image stitching. Stitchy is a robust software solution that stitches large imaging datasets 2.5-5.5 times faster than leading commercial software solutions. Stitchy enables seamless integration with downstream image analysis pipelines by eliminating intermediate file formats.
Translucence Biosystems labels and characterizes bile duct morphology, providing insights into liver pathologies and the impact of therapeutic interventions on bile duct architecture.
Browse actionable insights generated by the
Liver Bile Ducts and Tubular Structures
service
The left image is the raw CK7 signal from a P7 mouse liver, while the right highlights the bile duct network segmented by the Translucence Teravoxel Toolkit (3TK) software.
Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
Whole mouse liver stained with Smooth Muscle Actin (SMA) in Cyan and Cytokeratin 7 (CK7) in green. Tissue clearing and staining for SMA and CK7 enables the use of Light Sheet Fluorescence Microscopy (LSFM) to visualize vasculature and bile duct networks – providing comprehensive insights into the 3D morphology of both targets.
Visualization of liver vasculature using Smooth Muscle Actin (SMA) in the whole mouse liver. Tissue clearing and staining for SMA enables the use of lightsheet imaging to visualize the vascular networks in the liver. This approach provides robust insights into vascular networks, often implicated in liver diseases. Tissue clearing provides more comprehensive insights compared to traditional 2D slice immunohistochemistry (IHC) methods. Sample preparation was performed using the Translucence Universal Tissue Clearing Kit. This NIH-funded kit is suitable for clearing various tissue types, including mouse brain, lung, liver, spinal cord, and more. Imaging was performed on the ZEISS Lightsheet Z.1 equipped with the Mesoscale Imaging System™.
Whole mouse liver stained with Smooth Muscle Actin (SMA) in Cyan and Cytokeratin 7 (CK7) in green. Tissue clearing and staining for SMA and CK7 enables the use of Light Sheet Fluorescence Microscopy (LSFM) to visualize vasculature and bile duct networks – providing comprehensive insights into the 3D morphology of both targets.
The Liver Bile Ducts and Tubular Structures service leverages next-generation 3D histology technologies including tissue clearing, light sheet imaging, and AI-powered quantification to provide comprehensive insights into morphological changes induced by genetic or pharmacological manipulations.
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