Translucence Biosystems presents research at AD/PD™ 2022, Alzheimer's & Parkinson's Diseases Conference

by Damian Wheeler
March 30, 2022

Dear Collegues,

Translucence Biosystems was honored to present at the AD/PD™ 2022, Alzheimer's & Parkinson's Diseases Conference. Please see our poster presentation entitled, "Brain-Wide Cellular Resolution Measurement of Neuronal Activity, Microglial Activation and β-Amyloid Deposition" below.


Recent advances in optical clearing and light sheet imaging have opened an exciting new avenue for brain-wide, cellular resolution immunostaining at the forefront of a dimensional shift from 2D to 3D histology. With access to the intricate anatomy of the whole intact brain, we are developing unbiased and complete pictures of brain pathology for pre-clinical studies.

Methods: With our optimized iDISCO-based clearing methods and our Mesoscale Imaging System for the ZEISS Lightsheet Z.1 microscope, we can image entire mouse brains in ~10 min. Further, our machine learning-enabled 3TK software identifies individual cells or protein aggregates throughout the brain and aligns them to the Allen Reference Atlas to produce a regionalized read-out of patterns across 100’s of brain areas.

Results: We have applied this technology to generate cellular resolution maps of neuronal activity by measuring the immediate-early gene (IEG) products Npas4 and cFos in response to both a light exposure task and in response to contextual fear conditioning. Further, we have optimized methods to identify and count microglia throughout the brain and perform regional analyses of microglial activation.

Importantly, we have used Alzheimer’s Disease model 5xFAD mice to develop assays to generate brain-wide signatures of β-Amyloid deposition. Our automated analysis of microglia and β-Amyloid co-staining allows for brain-wide quantification of plaque-associated microglia. Conclusions: These new methods for whole-brain, next generation 3D immunohistochemistry and anatomics are ideally suited to pre-clinical studies for unbiased, complete and anatomically precise mapping of the efficacy of CNS therapeutics. We thank NIMH for Grants R43MH119989 and R43MH122070.

Ask your question

Send us your questions and we will get back to you as soon as possible

Thank you! Your submission has been received!
Something went wrong while submitting the form.
Please, contact via us direct email info@translucencebio.comand

Recent news

June 30, 2021

Celebrating the Fourth with Neuroscience Fireworks

Credit for this work goes to an NIH-supported team that includes Ricardo Azevedo and Sunil Gandhi, at the Center for the Neurobiology of Learning and Memory, University of California, Irvine, and their collaborator Damian Wheeler, Translucence Biosystems, Irvine, CA. Azevedo is also an NIH National Research Service Award fellow and a Medical Scientist Training Program trainee with Gandhi.
Damian Wheeler
June 15, 2021

Translucence Biosystems presents at the 7th Annual BRAIN Initiative Investigators Meeting

Translucence Biosystems is honored to present at the 7th Annual BRAIN Initiative Investigators Meeting. Our presentation is entitled, "Measurement of Npas 4 and cFos for Brain-Wide Snapshots of Neuronal Activity" Access our presentation materials below.
Damian Wheeler
June 7, 2021

On the forefront of 3D histology | Translucence Biosystems featured in CALIT2’s Interface Magazine

The California Institute for Telecommunications and Information Technology at the University of California, Irvine Fall 2019 issue covers transformational tools. The article features the Mesoscale Imaging System™, developed by then Graduate Student in the dual M.D./Ph.D. program University of California, Irvine, Ricardo Azevedo and Associate Professor, Sunil Gandhi, Ph.D.
Damian Wheeler