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Neuronal Activity

Npas4 Induction

Our Translucence Teravoxel ToolKit (3TK) software uses AI-powered workflows to identify individual Npas4(+) neurons throughout the cleared and stained intact mouse brain.

Each brain is computationally warped to the Allen Reference Brain and neurons are registered to an anatomical map to determine Npas4 expression metrics across 100’s of brain regions.

We have collapsed the hierarchical annotations into larger regions to demonstrate brain-wide Npas4 expression levels in 14 brains from mice exposed to light for various durations after housing in the dark for 24h.

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